ChimeraUGEM WebGUI

Input Sequences

Provide your proteins to optimize in FASTA format.

Maximum file size: 50 MB

Reference Genome

Select or upload a reference genome.

Maximum file size: 50 MB

Parameters

Input Sequences

Provide your nucleotide sequences in FASTA format.

Maximum file size: 50 MB

Reference Genome

Select or upload a reference genome.

Maximum file size: 50 MB

Parameters

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About the Algorithm

The ChimeraUGEM WebGUI is a tool for the analysis of gene sequences (coding and non-coding), as well as the design of protein coding sequences for optimized expression, based on the Chimera algorithms (Zur and Tuller, 2015; Diament et al., 2019, Burghardt and Tuller, 2025 (in submission)).

How to use

  1. Provide proteins/genes to optimize or analyze
  2. Select a reference genome or upload a custom one
  3. Set parameters, reasonable default values are provided. Check the tooltips for information about each parameter.
  4. Results are provided as FASTA (optimized sequences) or CSV (ChimeraARS scores) files

Download

Source (Python)